A research study was undertaken to determine the requirement for initiation and multiplication rate of axillary buds of mature tree of Mulberry. Axillary buds derived from actively growing young shoots of 6 years old Morus alba were used to initiate the culture. Successful bud shoot induction and shoot elongation was achieved on MS media with 5µM BAP concentration. High multiplication rate i.e.12 micro shoots per culture was obtained with 15µM BAP. After 4-5 weeks the shoots developed roots in the same media without adding any rooting hormones. These plantlets showed a good growth response in earthen pots and 85% survival of plants in soil was recorded.