Numerous common human disorders, including cancer, high blood pressure, high cholesterol, inflammatory bowel diseases, obesity, and oral health issues, have been linked to changes in the composition and activity of the gut microbiota. This review aimed to highlight the significance of probiotics due to their positive impacts on gut microbiota, overall human health, cancer prevention, and production of bioactive compounds. Therefore, modifying the gut microbiota's balance can be highly beneficial for maintaining the human health. Probiotics are live microbial supplements, when consumed with meals; they help the host by harmonizing his/ her intestinal microflora. Supplements containing these good bacteria can be used up, or they can be added to dairy-free or non-dairy foods and drinks. Probiotics are mainly categorized into two main genera; mainly Lactobacillus spp. and Bifidobacterium spp. Probiotics are vital for maintaining a healthy microbiota; especially in cancer patients. They enhance the production of anticancer enzymes, apoptosis, autophagy, immunology, and other processes. Additionally, probiotics produce various bioactive molecules such as short-chain fatty acids, enzymes, vitamins, and exopolysaccharides with antibacterial attributes. Consequently, probiotics are utilized in food technology to develop the functional foods that promote the human health.