Hematological analysis of blood of Rita rita, showed significant differences between total erythrocytes count, hemoglobin concentration and erythrocyte sedimentation rate of the two sexes. Male fish had total erythrocytes 5.05 ± 0.5 × 106 and hemoglobin content 11.5 ± 1.5 g/in ml compared to 4.04 ± 0.5 × 106 and 7.3 ± 1.3 g/in ml in the female. The female fish had significantly higher values of ESR in comparison of male. It may be due to different plasma viscosity and specific gravity of the erythrocyte is responsible for different ESR values.
Short Communication · Volume 50, Issue 5, 24 August 2018 · Open Access
Male Catfish Rita rita has higher Haemoglobin Concentration than Female Fish
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Affiliations & Notes ⌄
- 1Department of Aquaculture and Fisheries, Faculty of Bio-Sciences, Shaheed Benazir Bhutto, University of Veterinary and Animal Sciences, Sakrand 2Department of Freshwater Biology and Fisheries, University of Sindh, Jamshoro 3Department of Animal Production Technology, Shaheed Benazir Bhutto, University of Veterinary and Animal Sciences, Sakrand , * Corresponding author: dr_ntnarejo46@yahoo.com
Article Info ⌄
- Journal:
- Pakistan Journal of Zoology
- Published:
- 24 August 2018
From the article
- On the contrary female showed significantly higher values of erythrocyte sedimentation rate than male (4.5 and 5.5mm/h).
- It was observed that value of total erythrocytes count and hemoglobin concentration was found to be significantly higher in case of male (5.05 ± 0.5 × 106 and 11.5.0% and 4.04 ± 0.5 × 106 and 7.0%) then female.
Selected from the article's own text.
Abstract
Materials and methods
For hematological studies blood samples from fish of size ranged from 21.0-33.5cm and 139-500 g in length and weight for both the sexes, respectively, were collected during March-May 2016 from Indus River near Jamshoro Sindh, Pakistan. Total 100 samples were procured and preserved for subsequent studies. Samples for blood analysis were obtained through heart puncture by sterilized syringe.
Hemoglobin was enumerated as recommended through Sahli hemogloblenometer and expressed in g% in broad day light from lower to the top of the measuring tube through graduation mark of miniscus. The rate of red blood sedimentation (ESR) was measured by the distance erythrocyte had sedimented in scale from top of the hematocrit tube and were enumerated as mm/h. The total erythrocyte and leucocytes counts were made with the Neubauer counting chamber.
Results and discussion
Table I show the various hematological parameters of the male and female fish. Total erythrocytes count and hemoglobin concentration was found to be higher in male compared to female (5.05 ± 0.5 × 106 and11.5 g/100 ml and 4.04 ± 0.5 × 106 and 7.3 g/100 ml, respectively). On the contrary female showed significantly higher values of erythrocyte sedimentation rate than male (4.5 and 5.5mm/h). Rest of the parameters showed no significant variation between the sexes.
Table I.- Hematological indices of male and female catfish, Rita rita from Indus River near Jamshoro.
| Parameters | Male (n=50) | Female (n=50) | |
| Mean (Range) | Mean (Range) | ||
| Length (cm) | 27.2 ± 5.52 (21.0 - 33.5) | 27.2 ± 5.52 (21.0 - 33.5) | |
| Weight (g) | 319.5 ± 35.02 (139 – 50) | 319.5 ± 35.02 (139 – 500) | |
| Hb (g/100 ml) | 11.5a1 ± 1.52 (10.0 - 13.0) | 7.3b1 ± 1.32 (6.0 - 8.6) | |
| ESR (mm/h) | 4.5b1 ± 0.52 (4 – 5) | 5.5a1 ± 0.52 (5 – 6) | |
| TEC (× 106) | 5.05a1 ± 0.52 (4.5 - 5.1) | 4.04b1 ± 0.52 (3.5 - 4.5) | |
| TLC (×103) | 28.05a1 ± 0.52 (27.5 - 28.1) | 28.5a1 ± 0.52 (27.5 - 28.5) | |
| Differential count (%) | |||
| Large Lymphocytes | 2.5a1 ± 0.32 (2.3 - 2.8) | 2.5a1 ± 0.32 (2.3 - 2.8) | |
| Small Lymphocytes | 69a1 ± 1.02 (68.0 -70.0) | 69a1 ± 1.02 (68.0 - 70.0) | |
| Thrombocytes | 14.5a1 ± 1.52 (12.0 - 16.5) | 14.5a1 ± 1.52 (12.0 - 16.5) | |
| Monocytes | 1.5a1 ± 0.042 (1.2 - 1.6) | 1.5a1 ± 0.042 (1.2 - 1.6) | |
| Neutrophils | 24.5a1 ± 1.02 (23.5 - 25.5) | 24.5a1 ± 1.02 (23.5 - 24.5) | |
1Figures in the same row having same superscripts are not significantly (p˂0.05) different when compared on the basis of Duncan’s new Multiple Range test. 2Standard deviation.
Narejo et al. (2001) recorded hemoglobin concentration was higher in male (9.65% of blood and female 7.95%) in Monopterus cuchia and in male Pangasius sutchi, Rashid et al. (2002) recorded the range of Hb 6-10% with a mean of 8.45% of blood from Bangladesh. Willam et al. (2016) studied the hematological difference in African catfish Clarias gariepinus reared in pond and river from Nigeria Ranged of Hb were found to between 12.12-12.94% in both environments. Deghayem et al. (2017) and Odo et al. (2017) observed Hb values in African catfish Clarias gariepinus from Saudi Arabia. The ranged of hemoglobin was between 11.83-13.67%. The above ranges are in accordance with the present study. It could be due to accessory breathing organ in the experimental fish.
Conclusion
It was observed that value of total erythrocytes count and hemoglobin concentration was found to be significantly higher in case of male (5.05 ± 0.5 × 106 and 11.5.0% and 4.04 ± 0.5 × 106 and 7.0%) then female. Erythrocyte Sedimentation Rate was found more in female (5.5mm/h and 4.5mm/h) than male. Leucocyte and differential count did not show significant difference between the sexes.
Statement of conflict of interest
Authors have declared no conflict of interest.
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