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Short Communication · Volume 2, Issue 12, Pages 652–656, 4 December 2014 · Open Access

Isolation of Antibiotic Resistant <i>Salmonella</i> Organisms from Aborted Cows and Emu Birds from District Ludhiana, Punjab, India

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Journal:
Advances in Animal and Veterinary Sciences
Published:
4 December 2014
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From the article

  • Salmonella could be isolated from nine samples (7 from uterine fluid of aborted cows and 2 from fecal swabs of Emu birds).
  • This indicates that Salmonella may also be associated with bacterial abortion and infertility in cattle.
  • We found resistance to certain antibiotics among Salmonella and suggest here some of the most effective antibiotics for use in the field.
  • Keywords | Salmonella, Abortion, Antibiotic Resistance, Cow, Emu.

Selected from the article's own text.

Abstract

Vimlesh Gupta, Hari Mohan Saxena
Department of Veterinary Microbiology, College of Veterinary Science, GADVASU, Ludhiana, India.

*Correspondence | Hari Mohan Saxena, College of Veterinary Science, GADVASU, Ludhiana, India; Email: drhmsaxena@gmail.com

Abstract
We investigated 102 samples (faecal swabs of live birds, spleen, caecum, liver and congested intestines of dead birds and uterine discharges, and diarrheal faeces of cattle, buffaloes and horses) for the presence of Salmonella organisms. Salmonella could be isolated from nine samples (7 from uterine fluid of aborted cows and 2 from fecal swabs of Emu birds). Identification of the isolates was done on the basis of cultural, staining and biochemical characteristics. All the 9 isolates were negative for oxidase and positive for catalase, motility indole lysine, and citrate utilization tests. The isolates were confirmed to be Salmonella by PCR using Salmonella genus specific oligonucleotide primers which yielded the desired amplicons of 496-bp. This indicates that Salmonella may also be associated with bacterial abortion and infertility in cattle. We found resistance to certain antibiotics among Salmonella and suggest here some of the most effective antibiotics for use in the field. The isolated organisms were found to be resistant to erythromycin and penicillin and sensitive to gatifloxacin, gentamicin, ciprofloxacin, norfloxacin, doxycycline, cefoparazone, ceftaxime, and oxytetracycline. Salmonellosis being a zoonosis, the findings are of public health significance as well.

Keywords | Salmonella, Abortion, Antibiotic Resistance, Cow, Emu

†Values in the parenthesis indicate percent positivity or negativity.

Our results have reconfirmed the earlier observa tions of widespread prevalence of antibiotic resistance among Salmonella and prompt for undertaking such surveillance from time to time in different regions to get a realistic picture of the problem of emergence of resistant pathogens and to enable selection of the most effective antibiotics for use in the field. Murugkar et al. (2005) have also reported varying degrees of resistance of their Salmonella isolates against doxycycline, ampicillin, amoxicillin, tetracycline, chlortetracycline, nitrofurantoin, chlortetracycline, kanamycin, cephalexin, nalidixic acid, chloramphenicol, trimethoprim, ciprofloxacin, gentamicin, enrofloxacin and norfloxacin. Irimie et al. (2010) isolated Salmonella cholerae suis spp. from liver and intestine which were resistant to all the antibiotics used. Resistance to multiple antibiotics could possibly be due to the misuse of antibiotics in the feed.

Based on the findings of the present study, we opine that Salmonella enterica is not only a typical enteric pathogen but it may also be a major cause of bacterial abortion in cattle.

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Isolation of Antibiotic Resistant <i>Salmonella</i> Organisms from Aborted Cows and Emu Birds from District Ludhiana, Punjab, India · ResearchersLinks