Eleven pomegranate varieties were tested against six isolates of Colletotrichum gloeosporioides under controlled conditions. Inoculated fruits were assessed on the basis of virulence of the isolates and reaction of a particular variety was expressed in Disease Reaction Index (DRI). There was considerable variation in virulence and suscep-tibility within isolates and varieties. Isolate Cg 86 was found to be virulent (DRI 7.2), Cg 81 as less virulent (DRI 2.87), and other isolates were moderately virulent irrespective of varieties. Fruit varieties Aarakta and Bhagwa were found to be highly susceptible as both displayed maximum mean DRI 10.38 and 8.56, respectively irrespec-tive of isolates. Resistant varieties Yarcud local and Bedana thin skin showed uniform resistant reaction against six isolates of the pathogen. The interaction between isolates x varieties was also statistically significant. Highest DRI (15.22) was recorded in the interaction between Bhagwa x Cg 85 and it was on par with Ganesh x Cg 86, Aarakta x Cg 82, Aarakta x Cg 84, Ruby X Cg 86, Patna 5 X Cg 86 and Patna 5 X Cg 82.