There are more than 100 types of fungi that can yield mycotoxins. They are secondary metabolites and their main producing agents are Aspergillus flavus, Aspergillus nomius and Aspergillus parasiticus that are famous for Aflatoxin B1, B2, G1, and G2. The present study was conducted to quantify the aflatoxins in bovine feed provided to cattle found in and around Lahore. Total 50 samples were collected from two different localities of Lahore and categorized in two groups: Group A (Raiwind, 30 samples), Group B (Bedian Road, 20 samples). All samples were analyzed for estimation of aflatoxins level using thin layer chromatography (TLC) method. The results showed that out of 50 samples, 20 (40 %) were fit as no level of aflatoxins detected in these samples. However, 30 (60 %) were positive for aflatoxins. 15(50 %) samples were highly contaminated and unfit. However, the other 15 (50 %) samples were within the tolerance range of aflatoxins. Aflatoxin B1 was detected in all 30 samples and aflatoxin B2 was noticed in only 10 samples. However, Aflatoxin G1 and Aflatoxin G2 were not detected in any sample. It was concluded that there is a need to regulate managerial practices to improve the quality of bovine feed.