Recently production of virus-free potato minitubers is critical. For this purpose, infected tubers with potato leafroll virus (PLRV) or potato virus x (PVX) from two cultivars Spunta or Lady Rosette as demonstrated by serological detection were used as a source for virus-free minitubers production. This achived by subjecting tubers directly to thermotherapy treatment at 36ºC, 37ºC or 38ºC for three weeks which resulting in 50%, 70% or 80% of PLRV-free tubers and 0%, 16.6% or 33.3% of PVX-free tubers respectively. Moreover, no effects on the survival rate of tubers were detected. High percentage (93.1% or 76%) of PLRV- or PVX-free plantlets was achieved with meristem tip excision (0.1-0.2mm) from thermo-treated tubers at (38ºC) with survival rate 96.6% or 83.3% respectively. DAS-ELISA method was applied to the tubers directly, in vitro cultures and young acclimatized plantlets to detect these viruses. After direct transplant of in vitro virus-free plantlets in greenhouse. The statistical analysis showed that cultivars and ratio of bed components and their interaction had insignificant (P>0.05) effects on minituber number and minituber weight. However, ratio of bed components and interaction with cultivars were significantly (P<0.05) affected on minituber diameter. Maximum minituber diameter (13.64 mm) was recorded for Lady Rosette cultivar treated with Vermiculite + Sand (4:1), followed by Spunta cultivar (13.53 mm) applied with Vermiculite + Sand (4:1). Pots received Vermiculite + Sand (4:1), produced highest minituber diameter, while the least minituber was recorded for pots having Peat moss + Sand (4:1).