Neem oil was used in 0.5, 1.0, 2.0 and 3.0% concentrations. 100 percent mortality of shisham defoliator occurred in 3.0% concentration after one week of treatment. Maximum mortality of larvae was recorded as 33.33 % each in 2.0% and 3.0% concentration after 24 hours, 44.44% in 2.0% concentration after 48 hours and 56.11% in 3.0% concentration after 72 hours. Percent mortality was not significantly different among different concentrations of neem oil but was different significantly from control treatment.