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Research Article · Volume 50, Issue 4, 26 June 2018 · Open Access

Effect of Organic Acid Blend on Carcass Yield, Nutrient Digestibility and Tibia Ash during Starter Phase of Broiler Chicks

· · · · ·

Affiliations & Notes ⌄
  1. 1Department of Poultry Science, Faculty of Animal Husbandry and Veterinary Sciences, The University of Agriculture, Peshawar 2Department of Basic Veterinary Sciences, Faculty of Veterinary and Animal Sciennces, Gomal University, Dera Ismail Khan 3Department of Livestock and Poultry Science, Faculty of Veterinary and Animal Sciences, Gomal University, Dera Ismail Khan , * Corresponding author: asadzia2003@yahoo.com
Article Info ⌄
Journal:
Pakistan Journal of Zoology
Published:
26 June 2018
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From the article

  • The results indicated that supplementation of OA in a diet lowered in phosphorous content does not increase the tibial ash percentage.
  • Supplementation of OA at the rate of 2ml/L improved weight gain, feed efficiency, nutrient digestibility and enhanced mineralization of calcium and phosphorus in the tibia in broiler.

Selected from the article's own text.

Abstract

A total of 180 broiler was supplemented with organic acid (OA) at the rate of 0, (OA-0), 0.5 (OA-0.5), 1 (OA-1), 1.5 (OA-1.5) and 2 ml/l (OA-2) in drinking water during the starter period for 21 days. Feed intake was significantly (P<0.05) lower and weight gain and dressing percentage were significantly (P<0.05) higher in the OA-2 group, resultantly, the best feed conversion ratio (FCR) was recorded in the same group. Tibia dry matter, ash, calcium (Ca) and phosphorous (P) were significantly (P<0.05) higher in OA-2. On the other hand, fecal Ca and P decreased significantly (P<0.05) in OA-2. Mean dry matter and ash contents increased significantly (P<0.05) in OA-2, while crude protein, crude fibre, ether extract and metabolizable energy increased significantly (P<0.05) in OA-1.5 and OA-2. In conclusion, addition of OA in drinking water at the rate of 2 ml/L improved weight gain, feed efficiency, nutrient digestibility, and tibial mineralization of Ca and P in broiler.

Introduction

Birds are inevitably exposed to considerable stress under the modern system of poultry production , 2012a). The gastrointestinal tract of newly hatched chicks is immature and sterile. Development of GIT function and microflora occur as soon as the feed is ingested . Due to the concern of the antibiotic residues in meat, the use of antibiotics is banned as feed additives in routine in the recent years ; ; ; Zia ur Rehman et al., 2017). This leads to the use of chemical substances which are non antibiotic in nature (Khan et al., 2012b, c; ; Abudabos et al., 2018). Organic acids (OAs) are used as the best alternative, singly or in combination of many acids ; .

Short chain fatty acids (SCFAs) were amongst the OAs, judged as a good replacement for antibiotics ; . One such SCFA is butyric acid, which has more antibacterial activity ; . Butyrate obtained from the non starch polysaccharides fermentation is known as crucial for epithelial cells development, increase gut health and decrease occurrence of colon cancer in humans . However, in the caeca and intestines of young chicks, the level of SCFAs is quite low (van der Wielen, 2000). Some previous experiments showed positive effects of OAs on bone ash and mineral retention in pig and rainbow trout . In laying hen’s diet, the addition of OA showed a positive effect on quality of bones and eggshell . In the feed industry, to improve feed hygiene, various OAs are used , when the pH of digesta is low, the digestive enzyme and phytase activity is improved, and gastrointestinal functions are beneficially affected ; . Phosphorus, calcium and zinc retention was improved by citric acid in the tibia in broiler ; . The gastric pH can be lowered by adding OA into the feed and the pH of the digesta can be reduced by OA . The conversion of pepsinogen to pepsin is accelerated by low gastric pH, which enhances the rate of absorption of amino acid, minerals and protein . The objective of the present study is to find the effect of different levels of OA on the performance, digestibility and tibial strength of broiler during the starter period.

Materials and methods

The study was approved by the departmental committee on ethics and welfare of animals.

Experimental design

A total of 180 broiler birds (day old) was adapted for a period of one week and then divided into four groups designated as OA-0.5, OA-1, OA-1.5 and OA-2. Each group was further consisted of 3 replicates. The birds were fed with OA at the dose rate of 0, 0.5, 1, 1.5 and 2 ml/l in the group OA-0.5, OA-1, OA-1.5 and OA-2, respectively. The OA blend consisted of ortho phosphoric acid, formic acid and propionic acid. The feed comprised of maize, 60.7; soybean meal, 35.6; vegetable oil, 0.5; limestone, 1.4; dicalcium phosphate, 1.2; DL methionine, 0.15; salt, 0.4 and vitamin + trace mineral premix, 0.3. The calculated nutrient composition are protein 22% and poultry ME 2960 kcal/kg. Each kg of premix consisted: pyridoxine, 1 mg; folic acid, 0.4 mg; molybdenum, 0.32 mg; ethoxyquin, 25 mg; choline chloride, 60 mg; dI-α-tocopherol acetate, 4 mg; iodine, 0.2 mg; thiamine, 0.3 mg; Ca pantothenate, 3 mg; cyanocobalamin, 3 µg; biotin, 0.02 mg; Mn, 15 mg; Zn, 10 mg; iron, 4 mg; Cu, 1 mg; Co, 0.06 mg; Se, 0.02 mg; cholecalciferol, 0.018 mg; trans-retinol, 0.66 mg; menadione, 0.4 mg; riboflavin, 1.6 mg and niacin, 6 mg.

Performance traits

The estimation of the feed intake was done by providing a calculated amount of feed and weighed the daily feed refusal. Analysis of data was performed on a weekly basis. On a weekly basis, gain in body weight was calculated. On the first day of the week, the weight of the bird was calculated and then at the end of the week, the weight of the birds was measured to determine the gain in body weight. Feed conversion ratio (FCR) was calculated on a weekly basis and mortality was adjusted accordingly. The weight of gizzard, liver and heart was calculated as relative organ weight. From each replicate, two chicks were randomly selected which were first weighed and then slaughtered. Dressing percentage was determined by weighing the carcass.

Fecal collection

Specially constructed metabolic cages were provided for fecal collection. Six chicks were transferred for fecal collection from each replicate to the cages on day 17 of the experiment. Calculated amount of feed was provided till the last day of the trial in all the cages. Collection of feces was done on day 22. On a weekly basis, the weight of the chickens was taken. The trial was performed till the 22nd day.

Chemical analysis

Excreta samples were analyzed for nitrogen (Kjeldahl method; , CP (N × 6.25), dry matter (drying in oven at 103°C for 8 h), crude fibre , ether extract, calcium, phosphorus and ash . Metabolizable energy (ME) was determined with the help of bomb calorimeter as described by Sultan et al. (2015).

Measurements of tibia strength and ash content

From three chicks per group, the right tibial bone was taken. Cleaning of all the extra tissues was performed, and the tibia was placed in the refrigerator till analysis at 0oC. Weighing and drying of the tibia were done, followed by crushing and finally defattening for 48 h in refluxing ethyl ether in a Soxhlet apparatus. The tibia was dried in an oven in ceramic crucibles, and ashed at 550-600oC for 4-6 h. Expression of the ash content was done as a percentage of the fat-free and moisture-free tibia weight .

Table I.- Effect of OA on mean feed intake, weight gain, FCR and dressing percentage during starter phase.

Group

Feed intake (g)

Weight gain (g)

FCR (g/g)

Dressing percentage
Control

1063.18±5.51 a

578.31±4.63 d

1.72±0.006 a

59.28±0.21 d
OA-0.5

1047.71±3.84 ab

596.67±6.69 cd

1.71±0.011 ab

60.11±0.39 c
OA-1

1036.24±5.56 bc

606.33±7.68 c

1.69±0.014 b

60.45±0.55 bc
OA-1.5

1026.12±6.24 c

625.68±6.01 b

1.67±0.012 c

61.88±0.12 b
OA-2

1000.33±5.84 d

647.67±3.75 a

1.64±0.044 d

63.07±0.17 a
P-Value

0.0001

0.0001

0.0005

0.001

Mean values within a column differ significantly (P<0.05).

FCR, feed conversion rate

Data analysis

The standard procedure of Analysis of Variance (ANOVA) was used for the analysis of collected data using completely randomized design (CRD) as suggested by Steel and Torrie (1981) and Fisher LSD for significance difference. P values less than 0.05 were statistically considered significant.

Results

Mean feed intake, weight gain, FCR and dressing percentage are given in Table I. Feed intake was significantly (P<0.05) low and weight gain and dressing percentage was significantly (P<0.05) higher in the OA-2 group, resultantly, the best FCR was recorded in the same group.

Mean gizzard, liver, heart and mortality percentage is given in Table II. No significant difference was found in these parameters between the control and treated groups. Tibia dry matter, ash, calcium (Ca), phosphorus (P), fecal Ca and P of broiler chicks supplemented with OA blend is given in Table III. Tibia dry matter, ash, Ca and P were significantly (P<0.05) higher in OA-2. On the other hand, fecal Ca and P decreased significantly (P<0.05) in OA-2.

Mean nutrient digestibility in broiler chicks supplemented with OA blend is given in Table IV. Mean dry matter and ash contents increased significantly (P<0.05) in OA-2, while crude protein, crude fibre, ether extract and metabolizable energy increased significantly (P<0.05) in OA-1.5 and OA-2.

Table II.- Effect of organic acid (OA) on mean weight of gizzard, liver, heart and mortality percentage of broiler during starter phase.

Group

n

Gizzard (g)

Liver (g)

Heart (g)

Mortality (%)
Control

3

38.36±0.41

54.49±1.21

10.81±0.16

6.66±3.33
OA-0.5

3

38.98±0.66

55.21±0.62

10.95±0.18

6.66±3.33
OA-1

3

39.24±0.40

55.76±1.25

11.12±0.12

6.66±3.33
OA-1.5

3

39.40±0.37

56.72±0.79

11.24±0.19

3.33±3.33
OA-2

3

39.74±0.76

58.26±1.14

11.40±0.26

3.33±3.33
P-Value

3

0.509

0.195

0.318

0.871

Mean values within a column differ significantly (P<0.05)

Table III.- Effect of OA blend (aciflex) on tibia dry matter, ash, calcium, phosphorus, fecal calcium and phosphorus of broiler chicks.

Group

n

TDM

TASH

TCAL

T PHOS

FCAL

FPHOS
OA-0

3

96.25±3.45c

47.90±2.11a

0.78±4.22b

8.15±0.44bc

0.85±0.19a

9.74±0.14a
OA-0.5

3

96.41±1.45c

47.57±1.76a

0.35±1.45a

9.91±0.12ab

0.79±0.18b

7.66±0.15ab
OA-1

3

96.65±6.8b

51.56±1.44a

1.55±2.77a

6.94±0.21c

0.71±0.12c

5.04±0.19b
OA-1.5

3

97.15±4.52ab

48.25±3.77a

1.52±1.66a

9.53±0.87ab

0.67±0.11d

6.46±0.17ab
OA-2.0

3

97.74±5.42a

49.26±2.11a

1.43±1.55a

11.53±0.11a

0.56±0.18e

5.87±02b

Mean values within a column differ significantly (P<0.05). TDM, tibial dry matter; TASH, tibial ash; TCAL, tibial calcium; TPHOS, tibial phosphorous; FCAL, fecal calcium; FPHOS, fecal phosphorous.

Table IV.- Effect of OA blend (aciflex) on mean nutrients digestibility in broiler chicks.

Group

n

DM

ASH

CP

CF

EE

ME
OA-0

3

66.82±0.50 d

46.10±0.73 d

52.14±1.63 c

17.29±0.54 c

58.06±1.18 c

2307.7±16.8 c
OA-0.5

3

69.95±1.08 c

50.63±1.72 c

54.72±1.19 bc

18.06±0.66 c

59.48±0.25 bc

2355.5±34.6 c
OA-1

3

70.76±0.63 c

52.99±0.42 bc

59.45±0.83 ab

20.85±0.67 b

61.60±0.34 b

2468.9±24.1 b
OA-1.5

3

73.52±1.16 b

55.88±0.70 ab

65.39±3.35 a

23.95±0.75 a

64.49±0.96 a

2584.6±34.7 a
OA-2.0

3

76.24±0.58 a

57.03±0.85 a

66.26±3.09 a

24.57±0.28 a

64.87±0.47 a

2637.7±4.08 a

Mean values within a column differ significantly (P<0.05); DM, dry matter; ASH, ash; CF, crude fibre; CP, crude protein; EE, ether extract; ME, metabolizable energy.

Discussion

In the present study, feed intake, weight gain and FCR were improved by the supplementation of OA in broiler. OA tends to increase proteolysis action and leads to improved protein digestibility . It was found that pH of the GIT is lowered by the OA and utilize the nutrients better . The findings of the current study are in line with the results of the previous studies ; . The growth promoting effects of OAs were linked to their ability to control the growth of potential pathogens in the gastrointestinal tract of animals . Thus, the digestive tract remains healthy, functions more efficiently and leads to more efficient absorption of nutrients. OAs improve energy and protein digestibility by decreasing microbial load and decreasing subclinical infection . E. coli and other harmful bacteria are decreased by OAs, which enhance the growth of the poultry birds . Inside the body, it also produces an acidic environment which causes more availability of the nutrients which results in better performance of the birds . Denli et al. (2003) noted that intestinal cell proliferation was caused by different OA and therefore for absorption, more nutrients are available. Samanta et al. (2010) showed that the nutrient availability is increased by the acidic condition which results in better performance. The non significant effect of the supplementation of OA in broiler in the present study on the weight of liver, heart and gizzard is in agreement with Denli et al. (2003), Cakir et al. (2008) and Adil et al. (2010).

Our findings of digestibility of nutrients are in agreement with Boling et al. (2001) who reported higher values of total digestible nutrients in the diet supplemented with OA. Ao et al. (2009) reported that the digestibility of DM and CP increases with citric acid.

According to Onyango et al. (2003), in poultry, to assess response of P availability, tibia ash is considered to be the most sensitive criterion. In our experiment, when corn soyabean meal diet having a P deficiency was supplemented with an OA, did not affect the tibia ash. The results indicated that supplementation of OA in a diet lowered in phosphorous content does not increase the tibial ash percentage. Liem et al. (2008) reported that when a diet deficient in P content is fed to broiler birds, the tibial ash increased when supplemented with citric, malic or fumaric acid. Our findings are in line with Nezhad et al. (2008) who reported that in laying hens, citric acid has a beneficial effect on tibia bone mineralization.

Our results are in contrast with Sacakli et al. (2006) who reported that in quails when a diet deficient in phosphorus content is supplemented with OA (formic + lactic, 2.5 g/kg of diet), the tibial ash was increased. This positive effect of OA may probably be attributed to an increased availability of calcium and phosphorus ; . It has been observed that OA causes improvement of Ca availability by competitive chelating of Ca and a reduction in the formation of insoluble Ca-phytase-complexes (Boling et al., 2001).

Conclusion

Supplementation of OA at the rate of 2ml/L improved weight gain, feed efficiency, nutrient digestibility and enhanced mineralization of calcium and phosphorus in the tibia in broiler.

Statement of conflict of interest

Authors have declared no conflict of interest.

References

  1. Abudabos, A.M., Alyemni A.H., Dafalla, Y.M. and Khan, R.U., 2018. The effect of phytogenics on growth traits, blood biochemical and intestinal histology in broiler chickens exposed to Clostridium perfringens challenge. J. appl. Anim. Res., 46: 691-695. https://doi.org/10.1080/09712119.2017.1383258[CrossRef]
  2. Abudabos, A.M., Alyemni, A.H., Dafalla, Y.M. and Khan, R.U., 2017. Effect of organic acid blend and Bacillus subtilis alone or in combination on growth traits, blood biochemical and antioxidant status in broiler exposed to Salmonella typhimurium challenge during the starter phase. J. appl. Anim. Res., 45: 538-542. https://doi.org/10.1080/09712119.2016.1219665[CrossRef]
  3. Abudabos, A.M., Alyemni A.H., Dafallah, Y.M. and Khan, R.U., 2016. The effect of phytogenic feed additives to substitute in-feed antibiotics on growth traits and blood biochemical parameters in broiler chicks challenged with Salmonella typhimurium. Environ. Sci. Poll. Res., 23: 24151-24157. https://doi.org/10.1007/s11356-016-7665-2[CrossRef]
  4. Adams, C.A., 2004. Nutricines in poultry production: Focus on bioactive feed ingredients. Nutr. Abstr. Rev. B, 76: 1-12.
  5. Adil, S., Banday, T., Bhat, G.A., Mir, M.S. and Rehman, M., 2010. Effect of dietary supplementation of organic acids on performance, intestinal histomorphology, and serum biochemistry of broiler chicken. Vet. Med. Int., 2010: 479485. https://doi.org/10.4061/2010/479485[CrossRef]
  6. Alhidary, I.A., Rehman, Z., Khan, R.U. and Tahir, M., 2017. Anti-aflatoxin activities of milk thistle (Silybum marianum) in broiler. World Pltr. Sci.J., 73: 559-566.
  7. Ao, T., Cantor, A.H., Pescatore, A.J., Ford, M.J., Pierce, J.L. and Dawson, K.A., 2009. Effect of enzyme supplementation and acidification of diets on nutrient digestibility and growth performance of broiler chicks. Poult. Sci., 88: 111-117. https://doi.org/10.3382/ps.2008-00191[CrossRef]
  8. AOAC, 1990. Official methods of analysis, 15th ed. Association of Official Analytical Chemists, Washington, DC.
  9. AOAC, 2000. Official methods of analysis. Association of Official Analytical Chemists, Washington, DC.
  10. Boling, S.D., Snow, J.L., Parsons, C.M. and Baker, D.H., 2001. The effect of citric acid on calcium and phosphorus requirements of chicks fed corn soybean organic acid and broiler performance 639 meal diets. Poult. Sci., 80: 783-788. https://doi.org/10.1093/ps/80.6.783[CrossRef]
  11. Brenes, A., Viveros, A., Arija, I., Centeno, C., Pizarro, M. and Bravo, C., 2003. The effect of citric acid and microbial phytase on mineral utilization in broiler chicks. Anim. Feed Sci. Technol., 110: 201-219. https://doi.org/10.1016/S0377-8401(03)00207-4[CrossRef]
  12. Brons, F., Kettlitz, B. and Arrigoni, E., 2002. Resistant starch and the butyrate revolution. Trends Fd. Sci. Technol., 13: 251-261. https://doi.org/10.1016/S0924-2244(02)00131-0[CrossRef]
  13. Cakir, S., Midilli, M., Erol, H., Simsek, N., Cinar, M., Altintas, A., Alp, H., Altintas, L., Cengiz, O. and Antalyali, A., 2008. Use of combined probiotic-prebiotic, organic acid and avilamycin in diets of Japanese quails. Rev. Med. Vet., 11: 565-569.
  14. Chaveerach, P., Keuzenkamp, D.A., Lipman, L.J.A. and Van Knapen, F., 2004. Effect of organic acids in drinking water for young broilers on Campylobacter infection, volatile fatty acid production, gut microflora and histological cell changes. Poult. Sci., 83: 330-334. https://doi.org/10.1093/ps/83.3.330[CrossRef]
  15. Chung, T.K. and Baker, D.H., 1990. Phosphorus utilization in chicks fed hydrated sodium calcium aluminosilicate. J. Anim. Sci., 68: 1992-1998. https://doi.org/10.2527/1990.6871992x[CrossRef]
  16. Denli, M., Okan, F. and Celik, K., 2003. Effect of dietary probiotic, organic acid and antibiotic supplementation to diets on broiler performance and carcass yield. Pak. J. Nutr., 2: 89-91. https://doi.org/10.3923/pjn.2003.89.91[CrossRef]
  17. Dibner, J.J. and Buttin, P., 2002. Use of organic acids as a model to study the impact of gut microflora on nutrition and metabolism. J. appl. Poult. Res., 11: 453-463. https://doi.org/10.1093/japr/11.4.453[CrossRef]
  18. Garcia, V., Catala-Gregori, P., Hernandez, F., Megias, M.D. and Madrid, J., 2007. Effect of formic acid and plant extracts on growth, nutrient digestibility, intestine mucosa morphology, and meat yield of broilers. J. appl. Poult. Res., 16: 555-562. https://doi.org/10.3382/japr.2006-00116[CrossRef]
  19. Garrido, M.N., Skjervheim, M., Oppegaard, H. and Sorum, H., 2004. Acidified litter benefits the intestinal flora balance of broiler chickens. Appl. Environ. Microbiol., 70: 5208-5213. https://doi.org/10.1128/AEM.70.9.5208-5213.2004[CrossRef]
  20. Hadorn, R. and Wiedmer, H., 2001. Feuerstein D. Effect of different dosages of an organic-acid mixture in broiler diets. Arch. Gefluegelk., 65: 22-27.
  21. Hayat, T., Sultan, A., Khan, R.U., Khan, S., Zahoor ul Hassan, Ullah, R. and Aziz, T., 2014. Impact of organic acid on some liver and kidney function tests in japanese Quails, Coturnix coturnix japonica. Pakistan J. Zool., 46: 1179-1182.
  22. Hernandez, F., Garcia, V., Madrid, J., Orengo, J., Catala, P. and Megias, M.D., 2006. Effect of formic acid on performance, digestibility, intestinal histomorphology and plasma metabolite levels of broiler chickens. Br. Poult. Sci., 47: 50-56. https://doi.org/10.1080/00071660500475574[CrossRef]
  23. Islam, K.M.S., Schaeublin, H., Wenk, C., Wanner, M. and Liesegang, A., 2012. Effect of dietary citric acid on the performance and mineral metabolism of broiler. J. Anim. Physiol. Anim. Nutr., 96: 808-817. https://doi.org/10.1111/j.1439-0396.2011.01225.x[CrossRef]
  24. Jin, L.Z., Ho, Y.W., Abdullah, N. and Jalaludin, S., 1997. Probiotics in poultry: Modes of action. World’s Poult. Sci. J., 53: 351-368. https://doi.org/10.1079/WPS19970028[CrossRef]
  25. Jongbloed, A.W., Mroz, Z., Van Der Weij-Jongbloed, R. and Kemme, P.A., 2000. The effects of microbial phytase, organic acids and their interaction in diets for growing pigs. Livest. Prod. Sci., 67: 113-122. https://doi.org/10.1016/S0301-6226(00)00179-2[CrossRef]
  26. Khan, R.U. and Naz, S., 2013. Application of probiotics in poultry production. World’s Poult. Sci. J., 69: 621-632. https://doi.org/10.1017/S0043933913000627[CrossRef]
  27. Khan, R.U., Chand, N. and Ali, A., 2016. Effect of organic acids on the performance of Japanese quails. Pakistan J. Zool., 48: 1799-1803.
  28. Khan, R.U., Naz, S., Javadani, M., Nikousefat, Z., Selvaggi, M., Tufarelli, V. and Laudadio, V., 2012b. The use of turmeric (Curcuma longa) in poultry diets. World’s Poult. Sci. J., 68: 97-103. https://doi.org/10.1017/S0043933912000104[CrossRef]
  29. Khan, R.U., Naz, S., Tufarelli, V. and Laudadio, V., 2012c. Potential applications of ginger (Zingiber officinale) in poultry diet. World’s Poult. Sci. J., 68: 245-252. https://doi.org/10.1017/S004393391200030X[CrossRef]
  30. Khan, R.U., Naz, S., Nikousefat, Z., Selvaggi, M., Laudadio, V. and Tufarelli, V., 2012a. Effect of ascorbic acid in heat-stressed poultry. World’s Poult. Sci. J., 68: 477-490. https://doi.org/10.1017/S004393391200058X[CrossRef]
  31. Khan, R.U., Naz, S., Nikousefat, Z., Tufarelli, V., Javadani, M., Rana, N. and Laudadio, V., 2011. Effect of vitamin E in heat-stressed poultry. World’s Poult. Sci. J., 67: 469-478. https://doi.org/10.1017/S0043933911000511[CrossRef]
  32. Leeson, S., 2007. Vitamin requirement: Is there basis for re-evaluating dietary specifications? World’s Poult. Sci. J., 63: 255-266. https://doi.org/10.1017/S0043933907001444[CrossRef]
  33. Liem, A., Pesti, G.M. and Edwards, Jr. A.H.M., 2008. The effect of several organic acids on phytase phosphorus hydrolysis in broiler chicks. Poult. Sci., 87: 689-693. https://doi.org/10.3382/ps.2007-00256[CrossRef]
  34. Maenz, D.D., Engele-Schaan, C.M., Newkirk, R.W. and Classen, H.L., 1999. The effect of minerals and mineral chelators on the formation of phytaseresistant and phytase-susceptible forms of phytic acid in solution and in slurry of canola meal. Anim. Feed Sci. Technol., 81: 177-192. https://doi.org/10.1016/S0377-8401(99)00085-1[CrossRef]
  35. Martin, H. and Maris, P., 2005. An assessment of the bactericidal and fungicidal efficacy of seventeen mineral and organic acids on bacterial and fungal food industry contaminants. Sci. Aliments, 25: 105-127. https://doi.org/10.3166/sda.25.105-127[CrossRef]
  36. Nezhad, Y.E., Sis, M.N., Gholshani, A.A., Saedi, Y. and Aminvakili, R., 2008. The effects of combination of citric acid and microbial phytase on the concentration of some minerals of serum and parameters of mineralization of tibia in commercial laying hens. Asia J. Anim. Vet. Adv., 3: 375-380. https://doi.org/10.3923/ajava.2008.375.380[CrossRef]
  37. Onyango, E.M., Hester, P.Y., Stroshine, R. and Adeola O., 2003. Bone densitometry as am indicator of tibia ash in broiler chickens fed varying dietary calcium and phosphorus levels. Poult. Sci., 82: 1787-1797. https://doi.org/10.1093/ps/82.11.1787[CrossRef]
  38. Pakhira, M.C. and Samanta, G., 2006. Response of meat type quails to dietary probiotics. Ind. J. Poult. Sci., 41: 68-73.
  39. Rahman, Z., Naz, S., Khan, R.U. and Tahir, M., 2017. An update on the potential application of L-carnitine in poultry. World’s Poult. Sci. J., 73: 823-830. https://doi.org/10.1017/S0043933917000733[CrossRef]
  40. Sacakli, P., Sehu, A., Ergün, A., Genc, B. and Selcuk, Z., 2006. The effect of phytase and organic acid on growth performance, carcass yield and tibia ash in quails fed diets with low levels of non-phytate phosphorus. Asian-Aust. J. Anim. Sci., 19: 198-202. https://doi.org/10.5713/ajas.2006.198[CrossRef]
  41. Samanta, S., Haldar, S. and Ghosh, T.K., 2010. Comparative efficacy of an organic acid blend and bacitracin methylene disalicylate as growth promoters in broiler chickens: effects on performance, gut histology, and small intestinal milieu. Vet. Med. Int., 2010: 645150. https://doi.org/10.4061/2010/645150[CrossRef]
  42. Steel, R.G.D. and Torrie, J.H., 1981. Principles and procedures of statistics. McGraw-Hill Book Co. Inc, New York, NY.
  43. Sultan, A., Ullah, T., Khan, S. and Khan, R.U., 2015. Effect of organic acid supplementation on the performance and ileal microflora of broiler during finishing period. Pakistan J. Zool., 47: 635-639.
  44. Świątkiewicz, S., Koreleski, J. and Arczewska, A., 2010. Laying performance and eggshell quality in laying hens fed diets supplemented with prebiotic and organic acids. Czech J. Anim. Sci., 55: 294-306. https://doi.org/10.17221/207/2009-CJAS[CrossRef]
  45. van der Wielen, P.W., Biesterveld, S., Notermans, S., Hofstra, H., Urlings, B.A. and van Knapen, F., 2000. Role of volatile fatty acids in development of the cecal microflora in broiler chickens during growth. Appl. environ. Microbiol., 66: 2536-2540. https://doi.org/10.1128/AEM.66.6.2536-2540.2000[CrossRef]
  46. Vielma, J. and Lall, S.P., 1997. Dietary formic acid enhances apparent digestibility of minerals in rainbow trout, Oncorhynchus mykiss (Walbaum). Aqua Nutr., 3: 265-268. https://doi.org/10.1111/j.1365-2095.1997.00041.x[CrossRef]
  47. Vogtmann, H., Pfirter, H.P. and Prabucki, A.L., 1975 A new method of determining metabolisability of energy and digestibility of fatty acids in broiler diets. Br. Poult. Sci., 16: 531-534. https://doi.org/10.1080/00071667508416222[CrossRef]
  48. Youn, B.S., Nam, K.T., Chang, K.M., Hwang, S.G. and Choe, I.S., 2005. Effects of wood vinegar addition for meat quality improvement of old layer. Korean J. Poult. Sci., 32: 101-106.
  49. Zia ur Rahman, Chand, N. and Khan, R.U., 2017. The effect of vitamin E, L-carnitine and ginger on production traits, immune response and antioxidant status in two broiler strains exposed to chronic heat stress. Environ. Sci. Poll. Res., 24: 26851-26857.

Cited by 2 articles

In journals published by ResearchersLinks.

  1. Effect of Organic Acids Blend, Micro-Encapsulated Phyto-Essential Oils Individually or in Combination on Growth Performance, Gut Health and Nutrients Utilization of BroilersPakistan Journal of Zoology · 2022
  2. Growth Performance, Blood Lipid Profile, Relative Organ Weight and Meat Quality of Broilers Fed Corn-Soybean Meal Based Diets Containing Graded Levels of 1,3-DiacylglycerolPakistan Journal of Zoology · 2019

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