Peanut mottle virus (PMoV) was isolated from lentil cv. Giza 51. The infected plants feature severe mosaic on lentil cv. Giza51. mottling on cv. Giza9. mild mottle on cv. Giza4, yellow mild mottling on cv. Giza370, Seed transmission tests Of PMoV confirmed of the virus transmission through Lentil seeds. Peanut mottle virus was detected in the testas. cotyledons and embryo dissected from seeds of each Lentil cultivates. Electron microscopy of PMoV showed virus particles aggregates and degenerated mitochondria in cytoplasm of lentil leaf cells infected with PMoV. Effect of environmental factors and populations of insects on virus infection revealed a positive relationship was recorded between Insect incidence and percentage of virus infection during 2007 and 2009, meanwhile the temperature and relative humidity were lower in Behera than Fayoum. the percentage of virus infection was higher.