Sutures made with different materials are used in surgery for multiple reasons. These materials may be monofilament or multi filaments based on either natural or synthetic content. Mechanical and physical properties of sutures are crucial requirements to make an informed decision for their appropriate use in surgery. This study was aimed at determining and comparing the tensile strength of sutures made with silk and vicryl materials in straight and knot pull. An experimental study was conducted. Samples of sutures made with silk and vicryl used for surgery were collected from various pharmaceutical companies of Lahore. These were evaluated for their tensile strength by using DX Instron tester with two configurations. The results depicted that multifilament suture made with silk fiber had better tensile strength as compared to the vicryl in both straight and knot pull type.