Experiment on bioaccumulation of chromium (Cr) in the bodies of Catla catla, Labeo rohita and Cirrhinus mrigala was conducted from January through May, 2015. Water, sediment and fish samples were collected on monthly basis from upstream and downstream of head Trimmu, river Chenab. Throughout the study period, mmaximum Cr concentrations from upstream and downstream were recorded as 23.54± 3.43 mg/L and 21.12 ± 3.47 mg/L, respectively. Average metal concentration detected from sediment samples was 71.08±8.32 mg/L. Maximum Cr concentration 81.72±2.54 was recorded during February while minimum 61.24±1.23 mg/L during May. Among all the three fish species, maximum metal concentration was accumulated by Cirrhinus mrigala followed by Catla catla and Labeo rohita. Among fish organs, maximum Cr concentration 4.75±0.78 (µg/g) was recorded from liver whereas minimum 1.10±0.21 (µg/g) from muscles of the fish.