Oil extracted from the seeds of Datura stramonium with the help of soxhlet apparatus, using petroleum ether (40-600C) as solvent, was purified by treating it with activated charcoal and kieselghur. Yield, physicochemical characteristics and chemical composition of the oil were determined and the results obtained were compared with those of oil from Datura alba and other Datura species. It was concluded that this oil was of the same nature as those of the other Datura species and could be similarly used in medicine.