The biocalcification is considered as an environment friendly process that includes the precipitation of protective layer of calcium carbonate on the cement based structures or buildings. In the present study, the biocalcification capacity of Bacillus pasteurii was studied on the cement samples. The cement samples were treated with urea and calcium salt (calcium carbonate) that act as a source of calcium along with the microorganism under optimum culture conditions. Alkaline conditions produced by the activity of the enzyme urease allowed the reaction between calcium and carbonate ions that leads to the precipitation of the calcium carbonate. The water absorption test was performed to check the presence of a protective layer that provides resistance to water penetration and corrosion to cement. The samples treated with calcium and urea for one hour were found better as shown by less absorption of water compared with the untreated samples. Application of Bacillus pasteurii on the cement or the cement based materials has the potential to conserve such materials and increase their durability.