The purpose of this study was to observe the effects of heavy metals on proteins, chlorophyll content and growth of Eichhornia crassipes L. in different concentrations of industrial effluents of district Shiekhupura. Eichhornia crassipes was grown for 15, 30 and 45 days in different concentrations of the industrial effluents (5, 10, 15, 20 and 100%) prepared with tap water. All physico-chemical parameters in the different concentrations of the industrial effluents were recorded. The values of these parameters increased with increasing the concentrations of the wastewater. Heavy metals analyzed in the different concentrations of effluents were in the order of Cr > Cd > Pb. The growth parameters like shoot length, root length, fresh weight of leaves and roots, dry weight of roots and leaves, were recorded in the different concentrations of industrial effluents. The maximum growth was observed in 10 and 15% concentrations and minimum in 100% (industrial effluents). The uptake of metals Cr, Cd, and Pb was recorded after 15, 30 and 45 days of the experiment. The protein and chlorophyll content were reported in Eichhornia crassipes after 45 days. Amount of chlorophyll decreased with the increase in concentration of effluents at all stages while protein contents increased with the increase in concentration of effluents and with time.