Tyrosinase is a key enzyme in melanogenesis. Its high activity is associated with increased pigmentation causing skin disorders like freckles, melasma and black spots. Therefore, search for new tyrosinase inhibitors is desirable. In present study, methanolic (MeOH) extracts from leaves, fruit peel and pulp of Citrus bergamia (C. bergamia) and, leaves and fruit of Ficus carica (F. carica) were prepared which were further processed for fractional distillation using ethyl acetate (EA), n-hexane (n-Hx) and chloroform (CHCl3) preparing total 20 extracts aiming to test their anti-tyrosinase potential, in-vitro. Our results confirmed that all C. bergamia and F. carica crude extracts showed significant anti-oxidant activity with IC50 range of 384.2 ± 19.1 to 77.3 ± 10.0 μgmL-1, collectively. Moreover, significant anti-tyrosinase activity for C. bergamia (IC50 range = 4.1 ± 0.3 to 366.8 ± 36.5 μg mL-1) and F. carica (IC50 range = 156.5 ± 12.4 to 15.1 ± 2.9 μg mL-1) was found . Interestingly, C. bergamia MeOH-EA peel and C. bergamia MeOH-EA leaves extracts showed IC50 4.1 ± 0.3 and 6.1 ± 1.2 μg mL-1, respectively. Thus, C. bergamia MeOH-EA peel extract with lowest IC50 value among all the tested extracts, is proposed as potent candidate to control tyrosinase rooted hyperpigmentation in future.
Research Article · Volume 27, Issue 4, 31 May 2024 · Open Access
ANTI-TYROSINASE AND ANTI-OXIDANT POTENTIAL OF METHANOLIC EXTRACTS OF SELECTED Citrus bergamia AND Ficus carica PARTS
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Affiliations & Notes ⌄
- 1Department of Zoology, Mirpur University of Science and Technology (MUST), Mirpur-10250 (AJK)-Pakistan. 2Department of Medical Laboratory Technology, Riphah International University, Faisalabad, Pakistan.. 3Soil and Water Testing Laboratory, Mandi Bahauddin, Pakistan. 4Department of Medical Laboratory Technology, Government College University, Faisalabad, Pakistan. 5Department of Biochemistry and Biotechnology, The Islamia University of Bahawalpur, Bahawalpur-63100, Pakistan. 6Department of Phys
Article Info ⌄
- Journal:
- Pakistan Journal of Weed Science Research
- Published:
- 31 May 2024

Abstract
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