Sensitivity comparison between the RT-LAMP assay and the RT-PCR detection method. The detection limit of the RT-LAMP assay was determined based on real-time amplification plots and no visible amplification of GFLV, ToRSV and healthy samples as well as water control (A), Gel electrophoresis (b) and one-step RT-PCR (C). M. 100pb molecular marker (Biomatic-USA); Lane 1, total RNA 100ng/µl; Lane 2-6 tenfold serial dilutions of total RNA. The order of samples in panel C is the same as in panel B.