Estimation of TRAP activity of differentiated RAW264.7 cells. The cells were seeded at 12000 cells/cm2 density and differentiated for 72h. The control cells were fed with 0.05% DMSO supplemented medium. The positive control cells were fed with osteoclast induction medium (complete growth medium supplemented with 10ng/ml RANK-L and 0.05% DMSO). The CQ-H treated cells were fed with osteoclast induction medium containing 1, 10 and 100ng/ml CQ-H and Resveratrol treated cells were fed with osteoclast induction medium containing 0.002, 0.02, and 0.2 µg/ml resveratrol. TRAP activity was estimated after 72h of osteoclast differentiation induction. Data are represented as Mean ± SD of technical and biological replicates for each experiment. For statistical analyses, ANOVA with Dunnett’s test for multiple comparison was performed using GraphPad Prism (v 7.03). (P≤0.05; ns = not significant, * P≤0.05, **P≤0.01, ***P≤0.001, ****P≤0.0001).