Stained canine sperm under a confocal microscope (600x magnification). (A) High mitochondrial membrane potential, integral plasma and acrosome membrane. (B) High mitochondrial membrane potential, integral plasma membrane, and broken acrosome membrane. (C) High mitochondrial membrane potential, broken plasma membrane, and integral acrosome membrane. (D) Low mitochondrial membrane potential, integral plasma membrane, and broken acrosome membrane. (E) Low mitochondrial membrane potential, broken plasma membrane, and integral acrosome membrane. (F) Low mitochondrial membrane potential, broken plasma and acrosome membrane.