Multiple sequences alignment of cytochrome b protein in the model organism. Transmembrane alpha-helices in model organisms are highlighted in yellow colour. The conserved residues in archaea and eukaryotic are highlighted with turquoise colour. The eukaryotic conserved residues are highlighted with Gray colour. The dark yellow colour indicates the conserved residues in the high number of transmembrane alpha-helices model organisms. The MSA were performed through the online available Clustal Omega tool (https://www.ebi.ac.uk/Tools/msa/clustalo).