Visualization of host-virus protein-protein interactions in persistently infected cell cultures
In situ PLA for the indicated protein-protein interactions was performed in persistently BDV-infected cell cultures (C6BV), using uninfected C6 cells as negative control. Intense positive signals could be seen in the C6BV cells, especially in the nuclei, compared to the C6 cells. The number of signals per cell was quantified using Duolink ImageTool. The results of the quantifications are shown in Figure 5.