Methylation status of NGF gene in antler mesenchyme of female, male reindeer and sika deer. A: A schematic represents the distribution of the CpG site in the NGF gene and the analyzed sequence represents a 468 base pair fragment (positions −388 ~ +109) in the promoter region of NGF gene. B: Electrophoresis of BSP products of NGF. C: NGF methylation levels in antler mesenchyme of female, male reindeer and sika deer. For abbreviations and statistical detail, see Figure 3.