ELISA of protein content and statistical analysis of colonic muscular layer in the three groups of rats 7 days after modeling. A and B, comparisons of tau expressions at each phosphorylation site in the distal colonic muscular layer of NCG and OIC rats. C-F, the expressions of P-Ser396-Tau, P-Ser404-Tau, MOR and p-p38 were significantly increased in the colonic muscular layer of OIC rats compared with that of NCG and NSG (***P < 0.001).