Indirect serum stability response of SK vs HSA-SK.

(A) RP-HPLC analysis of purified Streptokinase showed single peak at 19 min; (B) RP-HPLC analysis of purified HSA-SK showed single peak at 10 min; (C) RP-HPLC analysis of SK taken after 4 hours of trypsin digestion showed that simple SK was almost completely digested in 4 hours; (D) RP-HPLC analysis of HSA-SK taken after 4 hours of trypsin digestion showed partial digestion in 4 hours and HAS-SK is more stable as compared to simple SK; (E) Tryptic digestion of both samples was performed and the difference in peaks was observed by RP-HPLC. HSA-SK showed decrease in rate constant for tryptic digestion as compared to SK.