(A) PCR amplification of NS3 and NS5A; Lane 1 DNA size marker (1 Kb), lane 2 amplified gene product NS3 (1.9 kb) lane 3 amplified gene product NS5A (1.3 KB). (B) Linearized purified plasmid constructs; Lane 1 and 2 linearized pcDNA/NS3 (5.4+1.9=7.3 kb) lane 3 DNA ladder (1 Kb) lane 4 and 5 linearized pcDNA/NS5A (5.4+1.3=6.7kb).