Expression level of hsa-mir-222-3p (A, B), hsa-let-7b-5p (C, D) and hsa-let-7f-5p (E, F) miRNA in canine mammary tumor was measured using RT-qPCR. C, E data from three technically replicated measurements were averaged and normalized to the internal RNU6B control. Log 2-fold change values were calculated for 10 dog mammary tumor samples (DMT). D, F, Relative expression of for all the three hsa-mir-222-3p miRNA using RNU6B normalized data of cancer (DMT) vs normal tissues (DNS) to calculate ∆Ct.