Effect of Calotropis procera on IL-6 production. At 8 (A), 12 (B) and 24 h (C) PHA stimulation of T-cells produced the IL-6 significantly (P<0.001) up to 357.1±47.48 pg/mL, 625.7 ± 8.08 pg/mL, and 772.9 ± 7.07 pg/mL respectively. While different doses of C. procera extract prevent the production of IL-6 by PHA stimulated T-cells. Data represents Mean±SD and analyzed by ANOVA with post-Tukey’s test (**P<0.01, ***P<0.001).