Total cell protein analysis of induced and uninduced transformed BL21 (DE3). (A) M is Page ruler plus prestained protein ladder, Lane 1-3 correspond to the total cell protein of induced BL21 (DE3) transformed cells and lane 4 corresponds to the total cell protein of uninduced BL21 (DE3) cells termed as negative control. (B) Qualitative analysis of purified rOmph protein (Lane 1, 2 and 3). (C) Immunoblotting of rOmpH protein. M: PageRuler protein ladder, lane 1-2: purified rOmph protein, lane 3: negative control, lane 4: histidine tag containing protein used as positive control.