SDS-PAGE analysis of the recombinant M protein solubilized using various concentrations of lauryl-dimethylamine oxide (LDAO). The protein was solubilized using 1% (panel A), 5% (panel B), and 10% (panel C) LDAO. For SDS-PAGE analysis, samples containing different quantities (10 µg, 15 µg, and 20 µg) of the total protein were loaded. It is evident that the target protein is best solubilized using 5% LDAO. Lanes 1, 4, and 7 represent the whole cell lysate, lanes 2, 5, and 8 depict soluble fractions, and lanes 3, 6, and 9 show insoluble fractions of lysed E. coli Rosetta 2(DE3) cells containing the expressed recombinant M protein. Arrows indicate the band corresponding to the target (His8-MBP-Matrix) protein (~ 83 kDa). Lane M depicts the mobility of a protein molecular weight marker for estimating the size of separated proteins.