Canine sperm stained with the combination of H324, PI, FITC-PSA, and JC-1 under a confocal laser scanning microscope (600x magnification). (a) Sperm with high mitochondrial membrane potential, intact plasma and acrosome membrane. (b) Sperm with high mitochondrial membrane potential, intact plasma membrane, and damaged acrosome membrane, and. (c) Sperm with high mitochondrial membrane potential, damaged plasma membrane, and intact acrosome membrane. (d) Sperm with low mitochondrial membrane potential, intact plasma membrane, and damaged acrosome membrane. (e) Sperm with low mitochondrial membrane potential, damaged plasma membrane, and intact acrosome membrane. (f) Sperm with low mitochondrial membrane potential, damaged plasma and acrosome membrane.