Agarose gel electrophoresis picture of PCR product showing the amplification of fragment of EEV gene at 958 bp. P: Control positive; L: DNA ladder; N: Negative fragment of LSDV in samples; Lane 1: Positive fragment of LSDV from skin nodules; Lane 2: Positive fragment of LSDV in samples taken from vaccine as positive control; Lane 3: Positive fragment of LSDV in samples taken from vaccine as positive control.