Genetic maps of plasmids for protein expression in E.coli. Panel a, plasmid pRSET/PAG1 encodes the recombinant protein boPAG1, with a decahistidine tag (10xHis tag) at the N-terminus. Panel b, plasmid pRSET/Trx-PAG1 encodes a fusion protein comprising E. coli thioredoxin (Trx) in the N-terminal part and boPAG1 in the C-terminal part. Genetic elements such as T7 promoter, ribosome binding site (RBS), T7 terminator, etc. are shown. Also shown are reading frames for the recombinant proteins boPAG1, Trx-boPAG1 and for a marker of antibiotic resistance (AmpR). Restriction sites used for cloning XbaI, NcoI, NheI are indicated.