A: The PCR amplification of target DNA fragment of 5S rRNA gene of E. vermicularis shows a 122 bp PCR product on agarose gel. M represents the marker of 100 bp Plus DNA ladder, starting from 100 with gradating band up to 3000 bp (Thermo Scientific). B: The second round of PCR amplification of target DNA fragment of 5S rRNA gene of E. vermicularis shows a 122 bp PCR product on agarose gel. M represents the marker of 100 bp Plus DNA ladder, starting from 100 with gradating band up to 3000 bp (Thermo Scientific). EV represents E. vermicularis specimens.