Purification and specificity verification of recombinant SeFABP. A, The purification results of SeFABP in inclusion bodies were analyzed by SDS-PAGE. Lane M, SDS-PAGE protein marker. Lane 1, supernatant after inclusion body dissolution and centrifugation. Lane 2, effluent after incubation of supernatant with Ni IDA. Lane3, elution component of 50 mm imidazole. Lane4-5, elution component of 300 mm imidazole. B, The quality and specificity of FABP were confirmed by SDS-PAGE and Western blot. Lane1, BSA (1.50 μg); Lane 2, SeFABP; M1, SDS-PAGE marker. M2, Western blot marker.