Characterization of blank CS-NPs and CS-LZ-NPs. (A1 and A2) particle size pattern of synthesized NPs showing (A1) size distribution of CS-NPs of 250 nm, (A2) size distribution of CS-LZ-NPs of 281 nm. (B1 and B2) TEM imaging of blank CS-NPs (B1) and CS-LZ-NPs (B2) showed consistent solid spherical nanoparticles without agglomeration and size ranged from 13.1 to 17.6 nm and 15.6 to 19.5 nm, Scale bar = 100 nm. (C) UV analyses of synthesized chitosan, chitosan lysozyme nanoparticles and lysozyme at wavelength range of 200-800 nm.