Expression and purification of CusR. A, total cell lysate (T), supernatant (S) and pellet (P) of un-induced and 0.1mM IPTG induced transformants grown at 37 °C. B, total cell lysate (T), supernatant (S) and pellet (P) of induced transformants, first given heat shock and then grown at 20 °C. C, dialyzed sample and FPLC fractions of CusR; dialysed sample (D), washout (W), Flow through (F), filtered sample (Filt). The subsequent lanes show FPLC fractions. Lane M: Bench mark protein unstained ladder.