Segregation analysis of T1 Cry2Aa brinjal plants. Randomly collected leaf samples from different plants of line E1, E3, E4, and E7 have been used to test the segregation by PCR. (A) E1 cry2Aa gene-specific PCR; (B) E3 cry2Aa gene-specific PCR; (C) E4 cry2Aa gene-specific PCR; (D) E7 cry2Aa. M, 1 kb ladder.