CRISPR/Cas9 induces indels in the Grp78 locus in GTCs. (A) Preliminary determination of the effect of gRNA (1–4) on GRP78 knockout in GTCs. (B-C) The expression of GRP78 in two knockout cell lines, KO-1 and KO-2. Tunicamycin (Tm) was added to the GTCs to stimulate GRP78 protein expression. (D) The position of the gRNA-1 target site and its sequence in the Grp78 locus in Capra hircus (top). Representative Sanger sequencing results of the PCR amplicons from the GTCs and KO-1 cells. The blue box indicates the target site sequence, and the insertion is highlighted in red (middle and bottom). *p<0.05 versus control group.