Maps of single, double and triple copy constructs prepared for the expression of human epidermal growth factor by multimerization of hegf gene. The expression cassette of about ~340bp was released by digestion with Bgl II and BamHI from single copy construct Figure 1A and inserted back to its BamHI linearized form for preparation of double copy construct i.e., pET28-EGF-C2 as shown in Figure 1B. The same expression cassette was inserted to the backbone of double copy construct, linearized with BamH1, for preparation of triple copy constructsi.e. pET28-EGF-C3 as shown in Figure 1C. Sequential steps repeated in same pattern can generate constructs with multiple copies of genes. Sequence maps were generated using SnapGene software version for Windows.