ALT suppresses stem-cell-like properties of glioma CSCs via suppressing YAP expression. (A) Western blot was used to examine the expression of stem cell markers (SOX2,OCT4,NANOG) and YAP, p-YAP in U87-spheroid cells treated with ALT for 96 h. (B) U87spheroid- shScramble cells and U87spheroid - shYAP cells were treated with ALT for 96 h, and expression of CD133,YAP, OCT4, SOX2 and NANOG was measured by Western blotting. (C) U87spheroid-shScramble cells and U87 spheroid - shYAP cells were treated with ALT (20μM) for 96 h, and expression of CD133 and YAP was observed by immunofluorescence (scale bar = 50 μm). (D) Comparison of mammosphere formation between U87-shScramble or U87-shYAP cells treated with ALT for 96h. (Scale bar =200 μm).