(A) Restriction digestion of pTZ57R/T plasmid cloned with ompH gene. The enzyme digesation confirmed the presence of gene of interest omph characterized by appearance of 1002 bps band and pTZ57R/T plasmid characterized by 2886 bps band (lane 1 and 2), M: O’Gene Ruler 1 kb DNA ladder. (B) the plasmid (pET-40b(+):omph) was digested with BamHI and SalI to futher confirm the cloning. The presence of two bands of 1002 and 6190 bps, confirmed the successful cloning of omphinpET-40(+) vector.