Fig. 4.

Effect of different concentrations of inhibitor UNC0642 administered for 48 h on relative expression of G9a (A), Cyclin D1 (B), fibroblast growth factor 1 (C) and Enolase 2 (D) genes determined by real-time PCR using HMBS as a reference gene for normalization and negative control (untreated) as calibrator. Compared with the respective controls, treatment with higher concentrations of inhibitor downregulated the expression of G9a, CCND1, FGF1 and ENO2 genes in both the cell lines. For statistical details see Figure 2.