4%-PAGE to show DNA-Protein interaction (Gel shift assay). Lane 1: the amplified bidirectional cusRS promoter (Prom), Lane 2: DNA Ladder Mix (SM006), Lane 3: BSA + cus promoter, Lane 4: promoter bound to CusR. Below it is shown multiple alignment of promoter cusRS sequence from K. pneumoniae KW, K. pneumoniae HS11286 (CP003785.1), K. pneumoniae NTUH-K2044 (AP006725.1), K. aerogenes KCTC 2190 (FO203355.1), K. variicola At-22 (CP001891.1), Raoultella ornithinolytica strain 18 (CP012555.1), and Citrobacter freundii strain P10159 (CP012554.1). Figure shows palindromic sequence of CusR binding box (Green color box), -10 box (yellow color box), -35 box (pink color box) conserved among different species of family Enterobacteriaceae. A/G in red font represents predicted transcription start site.