Efficient production of LP-5. Effect of temperature (A), pH (B), and different solvents on solubility of LP-5 (C). SDS-PAGE and western blotting of LP-5 following purification using Ni magnetic beads (D) SDS-PAGE finalized LP-5 protein refolding, dialysis and concentrated LP-5 protein by PEG 20,000 (E). Statistical analysis was performed using multiple t test via GraphPad Prism 6.0 (* p < 0.05, ** p < 0. 01, and p *** < 0.001).