ALT suppresses stem-cell-like properties of glioma stem cells via suppressing YAP expression. (A) U87 spheroid- oeScramble cells and U87 spheroid- oeYAP cells were treated with ALT for 96 h and expression of CD133, YAP, OCT4, SOX2 and NANOG was measured by Western blotting. (B) Immunofluorescence was used to detect the expression of CD133 and YAP in U87spheroid-oeScramble cells and U87spheroid -oeYAP cells (scale bar =50 μm). (C) Comparison of mammosphere formation between U87-oeScramble or U87-oeYAP cells were treated with ALT for 96 h. (Scale bar = 200 μm).