Comparative genome analysis of the three L. delbrueckii subsp. Bulgaricus isolates. (A) Roary matrix. Pan-genome analysis of three L. delbrueckii subsp. Bulgaricus isolates annotated genomes. Roary produced the gene presence/absence matrix. A total of 2284 protein-coding gene sequence clusters produced by roary are shown in the figure. The presence of the gene is represented by grey color while the absence is shown by white color. The numbers represent clusters in each assembled annotated genome. (B) Diagram of conserved genes per number of genomes. (C) Diagram of new and unique genes per number of genomes.