Lane 1, PCR products of LP 5-RCC1 cDNA. (B) Plasmid pET-32a(+)-LP-5  functional domain cDNA. M, DNA Marker DL8000; Lane 1, plasmid pET 32a(+) LP-5  extracted from JM109 cells; Lane 2, double restriction enzyme digestion of plasmid pET 32a(+) LP 5 RCC1 extracted from JM109 cells.