Gel electrophoresis of RPO30 gene based PCR assay for detection and differentiation of LSDV and SPPV.
a: Lane 1: 100bp DNA ladder, Lane 2: 50bp DNA ladder, Lane 3: SPPV (VSVRI), Lane 4: SPPV (RM 65 strain), Lane 5, 6, 7: SPPV (field strains), Lane 8, 9: LSDV (Ticks), Lane10, 11, 12, 13: LSDV (scabs), Lane 14: LSDV (buffy coat), Lane 15: LSDV (Ismailia strain), Lane 1: 50bp DNA ladder, Lane 2: 100bp DNA ladder
b: Lane 1: 100bp DNA ladder, Lane 3: LSDV (Ismailia strain), Lane 4: LSDV (skin nodules), Lane 5: LSDV (lymph aspirate), Lane 6:12: LSDV (skin nodules), Lane 13: SPPV (VSVRI), Lane 14: control negative