Influence of HGQ on the calorie restriction pathway in nematodes. Synchronized N2 nematodes were incubated at 20°C in 24-well plates with or without HGQ at the indicated concentrations, and the pharyngeal pump rate was measured on NGM plate at day 4 (A). Data are shown as mean ± SEM of four independent experiments with 40 nematodes assays for each treatment (A). Synchronized sir-2.1 mutant nematode VC199 were incubated at 20 °C in 96 wells plates with or without HGQ at the indicated concentrations, and the representative Kaplan-Meier lifespan curves are shown from three independent experiments with about 100 animals in each group (B).