ALT suppresses stemness properties of glioma. (A) Chemical structure of ALT. (B) U87 and U251 cells were treated with ALT for the 48 h and CCK8 assay was performed. (C) The cells were treated with ALT for 96 h and mammosphere formation was measured. Pictures were taken under a reverse microscope (scale bar = 200 μm). (D) U87- and U251-spheroid cells were treated with ALT for 96 h, and protein expressions level of glioma stem cells marker gene CD133 was analyzed by Western blotting.